C3142

Sigma

 

α-Chymotrypsin from bovine pancreas

(TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein

Synonym:TLCK-Chymotrypsin
CAS Number:9004-07-3
Enzyme Commission (EC) Number:3.4.21.1   ( BRENDA | IUBMB )
EC Number:232-671-2
MDL number:MFCD00130481

Description

Analysis NoteProtein determined by A1%/280
Biochem/physiol ActionsA serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the bond.
Other NotesView more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer
Preparation NoteDerived from New Zealand-sourced pancreas.
 TLCK treatment inactivates trypsin which may be present in chymotrypsin, without affecting the chymotrypsin activity.
Unit DefinitionOne unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Properties

typeType VII
formessentially salt-free, lyophilized powder
mol wtmol wt 25 kDa
storage temp.−20°C

Safety

Personal Protective Equipmentdust mask type N95 (US), Eyeshields, Faceshields, Gloves
Hazard CodesXn
Risk Statements36/37/38-42
Safety Statements22-24-26-36/37
WGK Germany1
RTECSGC3050000

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References

referenceGeiger, R. Meth. Enzym. Anal. 5, 99, (1984)
 Blow, D.M. Acc. Chem. Res. 9, 145, (1976)
 Rick, W. Meth. Enzym. Anal. 1, 1045, (1974)
 Ryan, C.A., Clary, J.J., Tominatsu, Y. Arch. Biochem. Biophys. 110, 175, (1965) Abstract
 Desnuelle, P. The Enzymes, (1960) 4, 93
MerckMerck 13,2282